# Executive Summary

# Maternal Preconception Study

## Restraint Stress Effect on Serum Corticosterone

# Introduction

The results of the analysis are presented using the following structure:

1. Searchable High‑Level Summary: Provides a brief overview of the key findings from our analysis with a table of effects
2. Visualisation, Descriptive Statistics and Diagnostics by Variable: Exploratory data analysis and model diagnostics for each behavioral measure
3. Searchable ROPE Analysis Table: Model diagnostics to check the fit of the models

# Effects of Interest

- No meaningful effects were found.

# Searchable High‑Level Summary

### Enter search terms in the column headers to filter the table below

Note: TD in the Higher column indicates a Trend Difference for the continuous variable.

# Visualisation, Descriptive Statistics and Diagnostics by Variable

### Bar Plot

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\(labels\)labels$x [1] “Time”

\(labels\)y [1] “Corticosterone (ng/mL)”

\(labels\)group [1] “ID”

\(labels\)colour [1] “color”

attr(,“class”) [1] “gg” “ggplot”

Corticosterone by Grouping Variables
Diet | n | Mean | SD | Min | Max | % of Zeros | Zero Flag
--- | --- | --- | --- | --- | --- | --- | ---
Control | 32.0 | 259.1 | 131.2 | 47.0 | 501.1 | 0.0 |
CR25 | 32.0 | 224.7 | 129.6 | 101.0 | 577.0 | 0.0 |
CRA | 32.0 | 193.7 | 66.9 | 112.4 | 421.0 | 0.0 |
CRV | 32.0 | 245.1 | 147.6 | 79.0 | 725.0 | 0.0 |
Warning symbols: * = >40% zeros in this group, ! = >20% zeros in entire sample Either flag triggers the use of a binary choice model.

### Summary Table

Model Summary
Family: lognormal
Parameter | Estimate | Std. Error | 2.5% CI | 97.5% CI | Type
--- | --- | --- | --- | --- | ---
Fixed Effects
Intercept | 5.449 | 0.161 | 5.132 | 5.769 | Fixed Effects
DietCR25 | −0.204 | 0.226 | −0.653 | 0.238 | Fixed Effects
DietCRA | −0.265 | 0.227 | −0.714 | 0.184 | Fixed Effects
DietCRV | −0.003 | 0.230 | −0.459 | 0.445 | Fixed Effects
Time | −0.001 | 0.002 | −0.004 | 0.003 | Fixed Effects
DietCR25:Time | 0.001 | 0.003 | −0.004 | 0.006 | Fixed Effects
DietCRA:Time | 0.001 | 0.002 | −0.003 | 0.006 | Fixed Effects
DietCRV:Time | −0.001 | 0.002 | −0.006 | 0.004 | Fixed Effects

### Posterior Check

$data # A tibble: 1,221 × 7 y_id y_name rep_id rep_label is_y is_y_label value 1 1 1 1 italic(y)[rep] ( 1 ) FALSE italic(y)[rep] 189. 2 1 1 2 italic(y)[rep] ( 2 ) FALSE italic(y)[rep] 296. 3 1 1 3 italic(y)[rep] ( 3 ) FALSE italic(y)[rep] 348. 4 1 1 4 italic(y)[rep] ( 4 ) FALSE italic(y)[rep] 216. 5 1 1 5 italic(y)[rep] ( 5 ) FALSE italic(y)[rep] 225. 6 1 1 6 italic(y)[rep] ( 6 ) FALSE italic(y)[rep] 268. 7 1 1 7 italic(y)[rep] ( 7 ) FALSE italic(y)[rep] 785. 8 1 1 8 italic(y)[rep] ( 8 ) FALSE italic(y)[rep] 781. 9 1 1 9 italic(y)[rep] ( 9 ) FALSE italic(y)[rep] 346. 10 1 1 10 italic(y)[rep] ( 10 ) FALSE italic(y)[rep] 851. # ℹ 1,211 more rows

\(layers\)layers[[1]] mapping: group = ~.data$rep_id, colour = yrep geom_line: na.rm = FALSE, orientation = NA stat_density: bw = nrd0, adjust = 1, kernel = gaussian, n = 1024, trim = FALSE, na.rm = FALSE, bounds = c(-Inf, Inf), orientation = NA position_identity

$layers[[2]] mapping: colour = y geom_line: na.rm = FALSE, orientation = NA, lineend = round stat_density: bw = nrd0, adjust = 1, kernel = gaussian, n = 1024, trim = FALSE, na.rm = FALSE, bounds = c(-Inf, Inf), orientation = NA position_identity

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$guides <Guides[0] ggproto object>

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### Convergence

Effect | Median | [95% HDI] | Direction Prob. | ROPE %
--- | --- | --- | --- | ---
Diet: Control vs CR25
Diet: Control vs CR25 | 0.13 | [-0.26, 0.52] | 76.00 | 14.85
Diet: Control vs CRA
Diet: Control vs CRA | 0.19 | [-0.18, 0.59] | 84.25 | 11.65
Diet: Control vs CRV
Diet: Control vs CRV | 0.05 | [-0.34, 0.42] | 61.75 | 20.25
Diet: CR25 vs CRA
Diet: CR25 vs CRA | 0.06 | [-0.34, 0.44] | 61.90 | 18.70
Diet: CR25 vs CRV
Diet: CR25 vs CRV | -0.08 | [-0.51, 0.30] | 64.95 | 18.20
Diet: CRA vs CRV
Diet: CRA vs CRV | -0.13 | [-0.51, 0.26] | 76.55 | 15.60

Effect | Median | [95% HDI] | Direction Prob. | ROPE %
--- | --- | --- | --- | ---
Time: Overall Change
Time: Overall Change 0 - 30 | 0.00 | [-0.06, 0.05] | 57.15 | 92.65
Time: Overall Change 30 - 60 | 0.00 | [-0.06, 0.05] | 57.15 | 92.65
Time: Overall Change 60 - 120 | -0.01 | [-0.12, 0.10] | 57.15 | 64.90

Effect | Median | [95% HDI] | Direction Prob. | ROPE %
--- | --- | --- | --- | ---
Time × Diet: Control - CR25
Time × Diet: Control - CR25 0 | 0.20 | [-0.21, 0.65] | 81.70 | 11.80
Time × Diet: Control - CR25 30 | 0.17 | [-0.23, 0.54] | 78.80 | 14.95
Time × Diet: Control - CR25 60 | 0.12 | [-0.25, 0.53] | 73.75 | 15.65
Time × Diet: Control - CR25 120 | 0.04 | [-0.47, 0.61] | 56.00 | 13.55
Time × Diet: Control - CRA
Time × Diet: Control - CRA 0 | 0.27 | [-0.16, 0.75] | 88.10 | 7.70
Time × Diet: Control - CRA 30 | 0.22 | [-0.16, 0.64] | 87.10 | 10.70
Time × Diet: Control - CRA 60 | 0.17 | [-0.23, 0.56] | 81.40 | 14.20
Time × Diet: Control - CRA 120 | 0.08 | [-0.44, 0.62] | 63.50 | 15.40
Time × Diet: Control - CRV
Time × Diet: Control - CRV 0 | 0.00 | [-0.49, 0.42] | 50.05 | 16.60
Time × Diet: Control - CRV 30 | 0.03 | [-0.38, 0.42] | 57.20 | 18.75
Time × Diet: Control - CRV 60 | 0.07 | [-0.35, 0.44] | 62.60 | 17.55
Time × Diet: Control - CRV 120 | 0.13 | [-0.40, 0.63] | 68.00 | 13.05
Time × Diet: CR25 - CRA
Time × Diet: CR25 - CRA 0 | 0.06 | [-0.41, 0.49] | 60.80 | 16.00
Time × Diet: CR25 - CRA 30 | 0.07 | [-0.33, 0.47] | 62.40 | 16.40
Time × Diet: CR25 - CRA 60 | 0.06 | [-0.34, 0.47] | 61.90 | 18.25
Time × Diet: CR25 - CRA 120 | 0.05 | [-0.50, 0.59] | 57.70 | 13.25
Time × Diet: CR25 - CRV
Time × Diet: CR25 - CRV 0 | -0.20 | [-0.71, 0.23] | 81.45 | 11.90
Time × Diet: CR25 - CRV 30 | -0.13 | [-0.54, 0.26] | 73.05 | 17.30
Time × Diet: CR25 - CRV 60 | -0.06 | [-0.44, 0.35] | 60.15 | 18.25
Time × Diet: CR25 - CRV 120 | 0.08 | [-0.49, 0.61] | 62.80 | 13.20
Time × Diet: CRA - CRV
Time × Diet: CRA - CRV 0 | -0.27 | [-0.74, 0.18] | 87.40 | 7.80
Time × Diet: CRA - CRV 30 | -0.19 | [-0.60, 0.21] | 83.25 | 11.90
Time × Diet: CRA - CRV 60 | -0.11 | [-0.52, 0.28] | 71.45 | 17.30
Time × Diet: CRA - CRV 120 | 0.03 | [-0.54, 0.49] | 53.90 | 14.35

Effect | Median | [95% HDI] | Direction Prob. | ROPE %
--- | --- | --- | --- | ---
Time: Control
Time: Control 0 - 30 | -0.02 | [-0.12, 0.08] | 63.40 | 61.15
Time: Control 30 - 60 | -0.02 | [-0.12, 0.08] | 63.40 | 61.15
Time: Control 60 - 120 | -0.04 | [-0.25, 0.16] | 63.40 | 33.35
Time: CR25
Time: CR25 0 - 30 | 0.02 | [-0.10, 0.13] | 66.35 | 57.90
Time: CR25 30 - 60 | 0.02 | [-0.10, 0.13] | 66.35 | 57.90
Time: CR25 60 - 120 | 0.04 | [-0.19, 0.25] | 66.35 | 31.15
Time: CRA
Time: CRA 0 - 30 | 0.03 | [-0.07, 0.13] | 68.70 | 58.70
Time: CRA 30 - 60 | 0.03 | [-0.07, 0.13] | 68.70 | 58.70
Time: CRA 60 - 120 | 0.05 | [-0.15, 0.26] | 68.70 | 30.75
Time: CRV
Time: CRV 0 - 30 | -0.05 | [-0.15, 0.05] | 82.80 | 47.65
Time: CRV 30 - 60 | -0.05 | [-0.15, 0.05] | 82.80 | 47.65
Time: CRV 60 - 120 | -0.10 | [-0.30, 0.10] | 82.80 | 22.75

# End of Document